VectoBac WG (also known as WDG) is a water-dispersible granule formulation of Bacillus thuringiensis subsp. israelensis (strain AM65-52) for control of mosquito larvae. The product has a potency of 3,000 International Toxin Units (ITU) per milligram against Aedes aegypti larvae. It is designed for use in aqueous spray mixes and for direct application. VectoBac WG was the first bacterial larvicide to complete the World Health Organization Pesticide Evaluation Scheme.
BioFuse™ technology allows VectoPrime™ to offer the industry’s widest single-brood application window (1st to 4th instar, or pre-flood) with the industry’s lowest rates for direct application to water, thus saving significant operational costs while improving application flexibility.
with highly specific activity against mosquitoes
|Not harmful to non-target populations|
(combines the dual modes of action from Bti strain AM65-52 and (S)-methoprene into a carefully selected ratio in every microparticle)
|Can treat all larval stages
Wider application window for early or late instars
Reduces number and volume of products needed; more warehouse space
|Quickly kills mosquito larvae||Only mosquito growth regulator formulation where results can be observed within 24 hours in the field (eliminates need to collect pupae to determine efficacy)|
|Controls all mosquito species||Application flexibility|
|Double “plus” potency and higher bulk density||Improved payload and lower application rates significantly reduce operational costs|
|Pre-flood application capacity
in select environments
|Reduces need to monitor flooding and wait for expected hatching|
|Standard size granule
(same as VectoBac® GS, VectoLex® FG and VectoMax® FG)
|Simplifies aircraft calibration and characterization|
BUILDING ON MOSQUITO ABATEMENT PROGRAM INSIGHT
Mosquito larviciding in many countries around the globe is generally based on short-residual biorational products using one of two active ingredients: Bacillus thuringiensis subsp. israelensis or (S)-methoprene. These larvicides can provide cost-effective solutions for control of single-brood floodwater mosquitoes. However, each of these active ingredients delivers optimal activity against different mosquito larval stages. As a result, multiple products are often used to manage changing scenarios.
In addition, many mosquito control programs manage multiple floodwater sites, but they have limited capacity to survey and treat all the sites once they are flooded. By the time many programs can reach a site, the larvae may have already progressed into late instars, and in some cases might have a mix of early and late instars. As a result, some programs have moved to pre-hatch products that have the residual capacity to treat multiple floods. However, in many cases, residual control of multiple floods is not needed. In these cases, a single, cost effective pre-flood application is sufficient to allow program managers the “breathing room” to focus on more immediate issues. The need for single-brood product flexibility—and the desire of programs to possess dual mode of action solutions—inspired Valent BioSciences Corporation to develop a next-generation tool for managing these public health problems.
Valent BioSciences’ experience in developing value-added products with combinations of active ingredients via its proprietary BioFuse™ technology led to the industry’s most flexible mosquito larval control solution for single-brood mosquitoes: VectoPrime™.
Valent BioSciences Corporation’s BioFuse™ technology combines Bti strain AM65-52 and (S)-methoprene in a specific toxin ratio into every microparticle of VectoPrime™. This technology offers mosquito control professionals the ability to take advantage of each active ingredient’s strengths, while significantly reducing the limitations that each possesses.
Single-brood Bacillus thuringiensis subsp. israelensis (Bti) products are most effective against early to mid instar larvae, and (S)-methoprene single-brood products are most effective against mid to late instar larvae (Fig 1). In either case, the most appropriate single-brood active ingredient to be used can be determined only after the habitat is sampled.
Differences in LC50s based on active ingredient and larval instar*:
* All figures extrapolated from the following published literature:
Fig 1, 2, 3: Phoochevin et al. 2008. Effects of cyromazin and methoprene on the developmental stages of Anopheles sirus, Aedes aegypti and Culex quinqefasciatus (Diptera: Culicidae).
Fig 1: Lacey et al. 1988. Host range and selected factors influencing the mosquito larvicidal activity of the PG-14 isolate of Bacillus thuringiensis var. morrisoni. J Am Mosq Control Assoc. 1988 Mar; 4(1): 39-43.
Fig 2: Boeckx et al. 2008. Communications in agricultural and applied biological sciences. Vol 73(3) 349-666.
Fig 3: Nayar et al. 1999. Laboratory evaluation of biotic and abiotic factors that may influence larvicidal activity of Bacillus thuringiensis serovar israelensis against two Florida mosquito species. J Am Mosq Control Assoc. 15(1): 32-42.
By combining Bti and (S)-methoprene in a single formulation, VectoPrime™ delivers effective control for multiple target mosquito species throughout all four instars. BioFuse™ technology ensures that each microparticle contains both AIs and they are delivered in appropriate ratios for consistent application and results.
Microparticles are quickly released on contact with water
Quick kills deliver visible proof that larvae are dead
Low application rates due to high potency and dual action
Dual action provides complete control of asynchronous broods
Easy to use—optimized for existing equipment and application strategies
VectoPrime™ offers abatement professionals superior operational flexibility with a wider application window across all instars and pre-flood. The ability to apply VectoPrime™ prior to flooding means you can plan ahead instead of having to react to changing environmental conditions, saving time and freeing up resources that would have been required for additional field surveillance and additional treatments. In addition, working with a single solution that kills all larval instars also allows you to streamline inventories and simplify replenishment.
In other words, you can start and end your week with the same solution.
With its revolutionary double “plus” Bti potency formulation and higher bulk density, VectoPrime™ can cut application rates in half for direct applications to water compared to current single-brood products, delivering more value per acre than conventional single-brood options. By doubling the effectiveness of every payload, you can save on fuel, maintenance, and labor costs.
The industry-standard granule size (same size as VectoBac® GS, VectoLex® FG and VectoMax® FG) streamlines calibration and characterization of application equipment. And the unique combination of AIs means you need only perform calibration and characterization once for your single-brood habitats. In addition, VectoPrime™ granules are non-abrasive, reducing attrition to application equipment when compared to sand-based products
|Clean Water||Polluted Water|
|VectoBac®||2.5–10 lbs/acre (2.8–11.2 kg/ha)||10–20 lbs/acre (11.2–22.4 kg/ha)|
|VectoPrime™||1.25–4.0 lbs/acre (1.4–4.5 kg/ha)||4.0–10.0 lbs/acre (4.5–11.2 kg/ha)|
*Estimated mean bulk density; note there are lot-to-lot variations in bulk density
|PRODUCT||GRANULE SIZE||BULK DENSITY||% INCREASE IN BULK DENSITY RELATIVE TO VECTOBAC® G|
|U.S. Standard Screen (Metric Conversion)||lbs/ft3 (kg/m3)*|
|VectoBac® G||5/8 mesh (2.4–4.0 mm)||25 lbs/ft3 (401 kg/m3)||—|
|VectoBac® GS||10/14 mesh (1.4–2.0 mm)||30 lbs/ft3 (480 kg/m3)||120%|
|VectoPrime™ FG||10/14 mesh (1.4-2.0 mm)||37 lbs/ft3 (597 kg/m3)||148%|
|POST-FLOOD APPLICATIONS||PRE-FLOOD APPLICATIONS|
|Suggested rate range:
1.25–10 lbs/acre (1.4–11.2 kg/ha)Use 1.25–4.0 lbs/acre against 1st–4th instar mosquito larvae. Use 4.0–10.0 lbs/acre when water is heavily polluted (e.g., sewage lagoons, animal waste lagoons), algae are abundant, and/or local experience suggests the need for higher rates. Retreat as needed based on local conditions.
|Suggested rate range:
10–20 lbs/acre (11.2–22.4 kg/ha)VectoPrime™ can be applied prior to flooding of mosquito larval habitats. Use 10–20 lbs/acre when up to 7 days pre-flood capacity is needed. Use 20 lbs/acre when a 7–14 day pre-flood application is needed. Retreat as needed. Consult your local Valent BioSciences representative for further advice on pre-flood applications with VectoPrime FG.